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Objective To evaluate the relationship between thyroid nodules combined with Hashimoto's thyroiditis(HT) and the risk of thyroid cancer.Methods This retrospective study included 1212 patients undergoing thyroidectomy in the Second Hospital of Tianjin Medical University from Jan.2010 to Jul.2017.The incidence of thyroid cancer in those thyroid nodules with and without HT based on the postoperative pathological results was compared.Results Among 843 patients of thyroid benign nodules,15.3％ cases were complicated with HT.Of all the patients with malignant tumors,365 cases were PTC,accounting for 96.5％ of all malignant tumors,and among whom,10.8％ were complicated with HT.Of the 169 cases of thyroid nodules combined with HT,23.7％ patients had PTC.For patients with thyroid nodules without HT,30.3％ had PTC.The difference had no statistical significance (x2=3.08,P＞0.05).Conclusion Thyroid nodules complicated with HT doesn't increase the incidence of thyroid cancer.
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Objective To investigate the proliferation and invasion of human pancreatic cancer cells after Stat1 gene silenced or enhanced and to explore the possible mechanisms.Methods BxPC-3 cells were divided to three groups: Stat1 group transfected with specific Stat1 plasmid, Si Stat1 group transfected with specific Stat1 small interfering RNA (siRNA), and control group.Western blot was used to detect the expression of Stat1, VEGF, MMP-2 and MMP-9 in BxPC-3 cells.MTT method was used to detect the proliferation of BxPC-3 cells.Transwell was used to detect the invasion of BxPC-3 cells.Results The proliferation and invasion were significantly enhanced in pancreatic cancer cells after Stat1 gene silenced, and the expression of VEGF, MMP-2 and MMP-9 was increased.The proliferation and invasion were significantly decreased in pancreatic cancer cells after Stat1 gene enhanced.Conclusions Stat1 inhibits the proliferation and invasion of pancreatic cancer cells.VEGF,MMP-2 and MMP-9 possibly play a role in this process.
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Objective To investigate the expression and clinical significance of transcriptional activator (STAT) 1 and matrix metalloproteinases (MMP)-2 in pancreatic cancer (PC). Methods The expressions of STAT1 and MMP-2 were detected by S-P immunohistochemical methods in 115 PC patients and whose age, tumor size, lymph node metastasis, TNM stage, differentiation and survival time were also collected. Results STAT1 protein was high expressed in 52 cases and MMP-2 was high expressed in 56 cases, which were significantly related with different clinical stages, tumor differentiation and lymph node metastasis in patients with PC (P<0.05). STAT1 expression was negatively correlated with MMP-2 expression (rs =-0.50, P<0.01). Lymph node metastasis, high level of clinical stage, low expression of STAT1 showed reduced postoperative survival time in patients with PC. Conclusion The lower expression of STAT1 and higher expression of MMP-2 play an important role in the progression of PC. The expression of STAT1 has practical value in the evaluation of prognosis in patients with PC .
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Objective To investigate the expression and significance of MMP-9 and Ki67 for predicting the progres-sion and prognosis of pancreatic cancer. Methods S-P immunohistochemical method was used to detect the expressions of MMP-9 and Ki67 in 100 pancreatic cancer tissue specimens. The relationship between the expressions of MMP-9 and Ki67 and patient age, tumor size, lymph node metastasis, tumor differentiation, clinical stages and prognosis were analyzed. Re-sults There were higher expressions of MMP-9 protein 46%(46/100) and Ki67 protein 53%(53/100) in 100 samples of pancreatic adenocarcinoma. And the expressions of MMP-9 and Ki67 were inversely associated with tumor differentiation, clinical stages, and lymph node metastasis of pancreatic cancer (P＜0.05). There were no significant differences in the ex-pressions of MMP-9 and Ki67 between patient age and tumor size. The expressions of MMP-9 and Ki67 were positive corre-lated (rs=0.405,P＜0.05). Moreover, the overall survival rates were correlated with patient age, lymph node metastasis, tumor differentiation and the expression of MMP-9, but no correlation with tumor size, clinical stages, and the expression of Ki67. Conclusion The expressions of MMP-9 and Ki67 were associated with pancreatic cancer progression. And the detection of expression of MMP-9 may have practical value in prognosis in patients with pancreatic cancer.
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Objective To evaluate our experience in the diagnosis and surgical management of papillary thyroid microcarcinoma(PTMC). Methods Clinical data of 42 PTMC cases were retrospectively analyzed. Results Twenty-five clinically nonpalpable PTMC were detected by high resohition thyroid uhrasonography preoperatively.The diagnosis of PTMC was established intraoperatively by frozen biopsy in 14 out of 19 cases undergoing this procedure.Of the 42 patients,30 underwent a lobectomy,and in 11 out of 30 patients supplemented level Ⅵ lymph node dissection was performed.None of these patients had recurrence during follow-up.The recurrence in three patients with multffocal lesions and undergoing incomplete resection were observed at follow.up.The mulifocality of PTMC and ipsilateral residual volume of the thyroid were two predicting factors that significantly influence the postoperative recurrence(P＜0.05,P＜0.01 respectively)in patients with PTMC. Candus-ions PTMC is usually occult and eludes correct preoperative diagnosis.Most PTMC are clinically nonpalpable and may be detected by hish resolution thyroid ultrasonography and diagnosed by frozen section during the operation.Surgery is the most important treatment of PTMC.Lobectomy plus level Ⅵ lymph node dissection is the therapy of choice for PTMC patients at the stage of cN0.